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Intermediate

Part:BBa_K1692033

Designed by: Kirsten Thompson   Group: iGEM15_Stanford-Brown   (2015-09-17)

RFP gDNA

This gene provides the intermediate for synthesizing gRNA targeting RFP (BBa_J04450). After transcription, this gRNA in conjunction with the Streptococcus pyogenes Cas9 enzyme will bind to and cleave the RFP plasmid. To learn more about the function of this gRNA, refer to http://2015.igem.org/Team:Stanford-Brown/CRATER

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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